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Simplify ELISA plate preparation with PIPETMAN® M96, a motorized 96-channel electronic pipette. Designed for high-throughput accuracy, it enables uniform dispensing across entire microplates while reducing variability and operator fatigue. With programmable protocols, ergonomic design, and compatibility with standard tips and labware, PIPETMAN M96 brings speed, precision, and reproducibility to every ELISA workflow.
Peptides, or short chains of amino acids linked together by covalent bonds, are a growing class of pharmaceutical drugs. With a multitude of purification methods available for peptide purification, it’s possible to use several techniques together to enhance the purity of the peptide. This article covers the range of purification and detection methods used to purify peptides from process-related impurities and product-related impurities.
Pharmaceutical peptides can be generated in a variety of ways. They can be generated by breaking down proteins to release peptides or they can be synthesized chemically, one amino acid at a time. This article covers synthetic peptide purification, focusing on purification by liquid chromatography-mass spectrometry (LC-MS).
The manual cyclization step in a peptide purification workflow is time-consuming and can easily become the rate-limiting step of the whole workflow, leading to delays in peptide production. Automating a drop-wise cyclization process using a liquid handler with a large rack capacity and incorporating automatic orbital shaking is an ideal solution to this workflow bottleneck. Learn more in this technical note.
When choosing a high performance liquid chromatography (HPLC) system, it's important to consider how your future needs may evolve from your current needs. In this article, you’ll learn about the advantages of modular HPLC systems when it comes to current experimental needs and how the ability to swap out HPLC components safeguards the future of your system and your research.
The Institute for Research and Innovation in Biomedicine (IRIB) focuses on developing innovative technologies for diagnosing, treating, and preventing diseases. In this case study, we explore how the researchers at IRIB are using preparative HPLC in their peptide purification drug discovery applications.
Preparative LC coupled with mass spectrometry (MS) is a well-established method for purifying target compounds. In this tech note, we demonstrate the use of a VERITY 271 LCMS system to perform mass-based purification of target peptides from a mixture. The system is controlled using TRILUTION® LC Software, which provides real-time and post-run MS and UV data.
The Coomassie (Bradford) assay is a well-established method for protein quantification that utilizes a commonly placed UV/Vis spectrophotometer. Streamlining the manual pipetting steps through
automated liquid handling results in less data variability and improvements in workflow efficiencies.
The type of pipette you use, and your pipetting technique will have a major impact on the accuracy and results of your assays. Almost every laboratory that conducts research will have a pipetting procedure that accurately measures the exact volumes when aspirating and dispensing liquids. However, if the workflow calls for pipetting dense liquids, making sure you’re using the correct pipette can ensure accuracy in your assays.
When pipetting small volumes, you'll need to take additional steps to improve your sampling accuracy. Here are five tips for improving your pipetting technique.
