Physicians need test kits to identify all of the genetic mutations of a condition at once so that they can initiate the right treatment. In this app note, we describe how the PIPETMAX automated liquid handler can be used within a BRCA MASTR Dx molecular diagnostic assay.

Reagents used in qPCR master mixes, including buffers, surfactants, and detergents, can negatively influence accuracy and precision, thereby increasing sample variability and decreasing data reliability. In this app note, we used PIPETMAX® to test whether the number of times a tip was pre-wet before transferring master mix affected qPCR performance.

The Bradford Assay is a common universal protein concentration determination technique. In this app note, you'll learn how the automation of this qualitative application shows comparability between manual and automated results for preparing a standard curve and samples for quantification of protein in solution.

In this application, NGS libraries were prepared from E. Coli DH10B genomic DNA (gDNA) and FAMlabeled adaptors using the NxSeq® DNA Sample Prep kit, comparing the manual method with an automated library prep protocol on the PIPETMAX. This application note describes the results of the ligation efficiency of manual library preparation versus automated library preparation.

In this application note we examine the limits of quantification and detection, repeatability, reproducibility, and recovery in food safety analytical testing. Automation with the GX-271 ASPEC® system provides a reproducible and reliable method for the isolation.

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has been used to create useful animal models for studying complex diseases, as well as correcting or inducing disease states within a cell. This technical note describes how PLATEMASTER and EXTRACTMAN can be used in the rapid enrichment and clonal selection of CRISPR modified cells.

This application note presents a new approach for the cleanup of DNA fragments from small volumes of liquid combining the innovative design of EXTRACTMAN® with the convenience of a DNA purification using MACHEREY-NAGEL’s proven NucleoMag® technology.

RNA-binding proteins (RBPs) are involved in many key cellular processes, but identification of the native binding partners (target RNAs) can be challenging. In this application note, we show that EXTRACTMAN can be used to efficiently and specifically enrich endogenously expressed ribonucleoprotein complexes.

EXTRACTMAN® improved isolation speed nine-fold and produced equivalent or better yields of purified DNA compared to tube-based isolation methods. In this application note, four DNA samples from two different organisms (mammalian and plant) were processed in parallel with no cross-contamination.

This application note describes a comparison of a conventional Co-IP protocol with an EXTRACTMAN® protocol using ESP technology. While the conventional Co-IP protocol did not pull down the protein-protein complex, EXTRACTMAN did, demonstrating the utility of ESP technology and EXTRACTMAN.